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Laboratory | Seed store | Projects

A small laboratory was set up and supplied with the basic equipment in 2003, and at the beginning of 2004 we started with the experimental phase of cultivating some selected rare and endemic plants of the Croatian flora (Ragusan knapweed, the Velebit carnation and others).
The advantage of the cultivation of plant  tissue in vitro is the relatively rapid reproduction of whole plants from a small sample (a pair or seeds or buds, no more).
In the first phase we germinate the seeds on artificial media that contain mineral salts, vitamins, sugar and plant hormones). In the second phase, the sprouts are transferred to a subcultivation medium.  Some parts of the sprouting plants are removed – such as the buds or parts of the stem – and are further reproduced (subcultivation, as it is called). Thus from only a single sprout, a hundred plants can be obtained by subcultivation. The young plants are then rooted on a special basal medium (gel or a liquid base) and transferred to sterilised soil.  Then comes the final phase – acclimatisation to external conditions of temperature and atmospheric humidity.

Velebitski karanfil in vitro

Until this moment the plants have grown in an ideal laboratory surrounding with constant temperature, lighting and hundred-percent relative humidity (the plants are inside closed test tubes or flasks). By the gradual process of getting them used to real-life conditions, the plants become hardier, more resistant and robust.   This is a very sensitive phase, and is frequently crucial for the further successful development of plants in vivo.  Experimental cultivation includes testing out nutrient bases with various different plant hormones, in different concentrations.   Media that give the best results will be used for later routine reproduction.
A large number of endemic, endangered and rare plant species grow in Croatia, and it is certainly worthwhile preserving them.  Such  ex situ programmes of protection can certainly help in this.

Aklimatizirana dubrovačka zečina

We introduce the plants into permanent cultivation (in laboratories and beds) and in this way ensure their survival (similar to the breeding of rare animal species in zoological gardens).  This is in particular important for those species the natural populations of which have suddenly shrunk because of the destruction of their habitats, climatic changes, human negligence and irresponsibility. It is our objective to organise occasional sales of plants  grown in this way, for the sake of public education, particularly that of the very young.

Isklijavanje velebitske degenije in vitro

The laboratory is run by Vanja Stamenković
and Dubravka Naumovski

Dubravka Naumovski




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last change: 10/10/2007
webmaster: Vanja N. Stamenkovic